Gandhi was seen winking during the high-pitched debate on Rafale fighter deal when AIADMK leader M Thambidurai was making his point.
The 48-year-old Congress leader was seated just behind Thambidurai but it was not clear as to whom he was winking at.
The video immediately went viral.
He had earlier stoked a controversy in July when he winked in the House, at that time during the debate on No-Confidence Motion.
At that time he had winked at his party colleague Jyotiraditya Scindia after hugging a seemingly unpleased Prime Minister Narendra Modi on the latter's seat.