Accusing Rajan of raising the interest rate to the detriment of small and medium industries, Swamy said the Governor should have known the "inevitable consequence of rising and high interest rate and his policy was wilful and thus anti-national in intent".
This is the second time in two weeks that Subramanian Swamy has written to Prime Minister Modi seeking immediate removal of Raghuram Rajan.
In the first letter written on 17 May, Swami had called Rajan 'mentally not fully Indian'.
In his second letter to Modi within a fortnight, Swamy alleged that despite holding a sensitive and very high government post, Rajan has been making mandatory trips to the US to renew his Green Card. He said the governor should have known the "inevitable consequence of rising and high interest rate and his policy was willful and thus anti-national in intent".
The BJP leader also claimed that Rajan has been sending confidential and sensitive financial information using an unsecured Chicago University email id and publicly disparaging the BJP government.
Swamy said that six allegations were "prima facie true" and called for an immediate termination of Rajan's services in the "national interest".
Swamy said that the RBI Governor's post "is very high in the Warrant of Precedence and requires a patriotic and unconditional commitment to our nation".
Rajan, he further alleged, is a member of US dominated Group of 30 which is engaged in defending America's dominant position in the global economy.