Meanwhile, the Rashtriya Janata Dal (RJD) broke out in protests outside the Assembly in protest.
The Chief Minister was faced vote of confidence in the Bihar Legislative Assembly, to prove his majority to form the state government.
The Bharatiya Janata Party (BJP) and Janata Dal (United) had issued whip to their legislators to vote in favour of the trust vote.
The RJD and the Congress had also issued a similar whip to their legislators.
Nitish led JD (U)-NDA government had a support of 132 MLAs.
According to the communique issued from the Raj Bhawan, Nitish had claimed the support of 131 members, including the BJP, two independents and legislators of BJP allies Hindustani Awam Morcha (HAM)-Secular and Rashtriya Lok Samata party (RLSP).
Opposition RJD has 80 MLAs, the Congress 27 and the CPI-ML 3.
Security arrangements were made in and around the assembly building ahead of the floor test.
Yesterday, Nitish and BJP leader Sushil Modi were sworn-in as Bihar Chief Minister and Deputy Chief Minister respectively, and former has been asked to prove his majority on the floor by Governor Keshari Nath Tripathi.
Nitish, on July 26 resigned as Bihar Chief Minister citing political differences with former ally, Lalu Prasad Yadav led Rashtriya Janata Dal (RJD), over corruption charges against the RJD supremo's son Tejashwi Yadav.
He, however, on Thursday, took oath as Bihar Chief Minister for the sixth time, marking a historic transition of the state from a Grand Alliance government to an NDA regime.
-With ANI inputs.